The role of labile iron pool in cardiovascular diseases.

Existence of an Erythropoitic labile iron pool

A FRET Probe for Ratiometric Fluorescence Imaging of Labile Iron Pools in Living Cells

Iron is an essential trace element for all living organisms, and it is also the most plentiful transition metal in the human body [14]. Due to the ability to cycle between multiple oxidation states, iron plays a key role in biological systems, spanning nucleotide synthesis to oxygen transport to electron transfer [5-7]. However, this same potent redox activity makes iron in unregulated forms po...

Repression of the heavy ferritin chain increases the labile iron pool of human K562 cells.

The role of ferritin in the modulation of the labile iron pool was examined by repressing the heavy subunit of ferritin in K562 cells transfected with an antisense construct. Repression of the heavy ferritin subunit evoked an increase in the chemical levels and pro-oxidant activity of the labile iron pool and, in turn, caused a reduced expression of transferrin receptors and increased expressio...

Repression of ferritin expression modulates cell responsiveness to H-ras-induced growth.

We assessed the role of the cell labile iron pool in mediating oncogene-induced cell proliferation via repression of ferritin expression. When HEK-293 cells, engineered to inducibly express either active (+) or dominant-negative (-) forms of the H-ras oncogene, were treated with antisense nucleotides to ferritin subunits they displayed (a) decreased ferritin levels, (b) increased labile iron po...

RED CELLS Repression of ferritin expression increases the labile iron pool, oxidative stress, and short-term growth of human erythroleukemia cells

The role of ferritin expression on the labile iron pool of cells and its implications for the control of cell proliferation were assessed. Antisense oligodeoxynucleotides were used as tools for modulating the expression of heavy and light ferritin subunits of K562 cells. mRNA and protein levels of each subunit were markedly reduced by 2-day treatment with antisense probes against the respective...